Synergistic anti-inflammatory activities of a new flavone and other flavonoids from Tephrosia hildebrandtii vatke.

Abstract

A new avone, named hilde avone (1) along with 7 other known avonoids were isolated from the aerial parts of Tephrosia hildebrandtii Vatke. Their characterisation was based on NMR and MS data analysis. The anti-in ammatory properties of the crude extract, isolated compounds and combination of the compounds were investigated in lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs). Treatment of the LPS-stimulated PBMCs with the isolated avonoids at a concentration of 100 µM signi cantly reduced the production of interleukins (IL1β, IL-2 and IL-6), interferon-gamma (IFN-γ), granulocyte macrophage-colony stimulating factor (GM-CSF) and tumour necrosis factor-alpha (TNF-α). It was also found that the combination of a avone and avanones exhibited remarkable synergistic anti-in ammatory e ects on the production of the cytokines.

Keywords: Fabaceae, Tephrosia hildebrandtii, avonoid, anti-in ammatory, hilde avone cytokine synergy