Effect of the leaf extract of bidens pilosa on haemostasis.

Abstract

Aims: To determine the effect of the leaf extract of Bidens pilosa on the rate of haemostasis and validate its traditional use application to fresh wounds. Study Design: Experimental Place and Duration of Study: The study was conducted at the Biology Department, Faculty of Science and Education, Busitema University and Nagongera Health Center IV laboratory between April and May 2019. Methodology: Different concentrations of the extract were applied to blood samples. Whole venous blood was collected by vein puncture in heparin tubes. The rate of clotting in presence and absence of the extract was determined. The experiment was replicated. Results: Increase in the concentration of the extract decreased the rate of haemostasis. Statistical analysis with a two-way ANOVA was significant, P = 0.02 at a 95% CI. Conclusion: High concentration Bidens pilosa leaf extract decreases the rate of haemostasis but may have other healing activities attributed to its historical and traditional use and application to fresh wounds. Keywords: Haemostasis; leaf extract; clotting; Bidens pilosa; ANOVA