Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola)

Abstract

Objective: To determine the phytochemical composition, antioxidant and anticancer ethanolic and water leaves extracts of Annona muricata (A. muricata) from the Eastern aUcgtiavnitdiae.s of Methods: Phytochemical screening was conducted using standard qualitative methods danifdfe rae nCth pi-hystqoucahreem gicoaoldsn. eTshse oafn ftiito xtiedsat nwt aasc tuivsietdy wtoa sa sdseitgenrm thinee dre ulastiinvge tahbe u2n, d2a-ndciep hoefn tyhl-e 2d-etpeircmryinlheydd ursaiznygl tahnrede rdeidffuecreinngt cpeollw leinr ems. ethods whereas the in vitro anticancer activity was Results: Phytochemical screening of the extracts revealed that they were rich in secondary clalacstosn mese,t aabnothlirtaeq cuoinmopnoeusn, dtasn snuicnhs, acsa radlkiaacl ogidlysc, ossaidpeosn,i npsh, etneorplse nanoidd sp,h fylatovsotneoroidlss., Tcootuaml pahriennso alincds in the water extract were (683.69依0.09) μg/mL gallic acid equivalents (GAE) while it was (372.92 e依x0t.r1a5c) tμ agn/md L47 G0.A51E μ ign/ mthLe GeAthEa nino ltihce eextthraancot.l iTch eex trreadcut.c Iinn gv iptroow aenr tiwoaxsid 2a1n6t. 4a1c tμivgi/tmy LIC in the water mg/mL and 0.9077 mg/mL for ethanolic and water leaves extracts of A. muricata respe5c0 twivaesl y2.. 0T4h5e6 ethanolic leaves extract was found to be selectively cytotoxic in vitro to tumor cell lines (EACC, Mit DhaAd a nnod cSyKtoBtRox3i)c w eiftfhe cICt o50n v nalouremsa ol fs p33le5.e8n5 cμegl/lms. L, 248.77 μg/mL, 202.33 μg/mL respectively, while The data also showed that water leaves extract of A. muricata had no anticancer effect at all tested concentrations. Conclusions: The results showed that A. muricata was a promising new antioxidant and anticancer agent