Biosynthesis of silver nanoparticles using hoslundia opposita aqueous leaf extract

Abstract

Nanotechnology provides an environmentally friendly green technique for synthesizing AgNPs using various parts of the plant. In this research, aqueous leaf extract of the H.opposita plant was used. 1mM AgNO3 solution was used as a reducing agent. The synthesized AgNPs gave maximum peak wavelengths ranging from 400-500nm which was a confirmation of their presence. Change of color was observed within 30min of the reaction which further confirmed their presence. Characterization of the AgNPs was done using the UV VIS spectrophotometer, FTIR and the SEM. The UV VIS spectra was measured at varying extract concentrations and time, and the absorbance of the AgNPs were seen to increase with increasing extract concentration and time. The 3g concentration showed the best maximum peak and the highest absorbance. The UV VIS absorption spectra were measured while varying reaction time from 0,1,24, and 48h and the best absorbance value was seen after 48h. FTIR of the leaf extract confirmed presence of –OH, -C=C-, O=C=O groups which were responsible for stabilizing and reducing the Ag+ to Ag0. The SEM image needed a powdered sample for better analysis. Further research is encouraged on the antibacterial activity of the H.Opposita biosynthesized AgNPs.